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cx43 shrna (Santa Cruz Biotechnology)

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Santa Cruz Biotechnology cx43 shrna
Effects of EPA on the expression of <t>Cx43</t> in tumor cells. (A) B16F10 cells were treated with EPA (0-100 μM) for 24 h. The number of cell was measured by the WST-8 assay. (B) The B16F10 cells were treated with of EPA for 24 h. The B16F10 cells were collected and measured for Cx43 by Western blotting. The Immunoblotting assay was repeated three times with similar results.

Effects of EPA on the expression of <t>Cx43</t> in tumor cells. (A) B16F10 cells were treated with EPA (0-100 μM) for 24 h. The number of cell was measured by the WST-8 assay. (B) The B16F10 cells were treated with of EPA for 24 h. The B16F10 cells were collected and measured for Cx43 by Western blotting. The Immunoblotting assay was repeated three times with similar results.

Cx43 Shrna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cx43+shrna+plasmid/connexin+43+shRNA+Plasmid/pmc06566740-50-28-14
Average 93 stars, based on 16 article reviews

cx43 shrna - by Bioz Stars, 2026-11

93/100 stars

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1) Product Images from "Eicosapentaenoic acids enhance chemosensitivity through connexin 43 upregulation in murine melanoma models"

Article Title: Eicosapentaenoic acids enhance chemosensitivity through connexin 43 upregulation in murine melanoma models

Journal: International Journal of Medical Sciences

doi: 10.7150/ijms.30889

Effects of EPA on the expression of Cx43 in tumor cells. (A) B16F10 cells were treated with EPA (0-100 μM) for 24 h. The number of cell was measured by the WST-8 assay. (B) The B16F10 cells were treated with of EPA for 24 h. The B16F10 cells were collected and measured for Cx43 by Western blotting. The Immunoblotting assay was repeated three times with similar results.

Figure Legend Snippet: Effects of EPA on the expression of Cx43 in tumor cells. (A) B16F10 cells were treated with EPA (0-100 μM) for 24 h. The number of cell was measured by the WST-8 assay. (B) The B16F10 cells were treated with of EPA for 24 h. The B16F10 cells were collected and measured for Cx43 by Western blotting. The Immunoblotting assay was repeated three times with similar results.

Techniques Used: Expressing, Western Blot

MAPK inhibitors reduced EPA-induced Cx43 expression. (A) The B16F10 cells were treated with EPA (0-100 μM) for 24h. The cells were lysed and protein expression of ERK, P38, JNK, P-ERK, P-P38, and P-JNK was examined. (B) After treatment of cells with inhibitor for p38 (SB203580) and JNK (SP600125) for 1h, The B16F10 cells were treated with EPA (100 μM) for 24h. The cells were lysed and protein expression of Cx43, P-P38, P-P38, JNK and p-JNK was examined. (C) EPA induced Cx43 transcriptional activity in B16F10 cells. The B16F10 cells transfected with luciferase gene under the control of Cx43 promoter were treated with EPA (0-100 μM) for 24 h. The transcriptional activity of Cx43 was determined by the luciferase reporter assay and is expressed as the fold of the relative luciferase activity relative to that in the control tumor cells. (n = 4, data are mean± SD. * P < 0.05, ** P < 0.01).

Figure Legend Snippet: MAPK inhibitors reduced EPA-induced Cx43 expression. (A) The B16F10 cells were treated with EPA (0-100 μM) for 24h. The cells were lysed and protein expression of ERK, P38, JNK, P-ERK, P-P38, and P-JNK was examined. (B) After treatment of cells with inhibitor for p38 (SB203580) and JNK (SP600125) for 1h, The B16F10 cells were treated with EPA (100 μM) for 24h. The cells were lysed and protein expression of Cx43, P-P38, P-P38, JNK and p-JNK was examined. (C) EPA induced Cx43 transcriptional activity in B16F10 cells. The B16F10 cells transfected with luciferase gene under the control of Cx43 promoter were treated with EPA (0-100 μM) for 24 h. The transcriptional activity of Cx43 was determined by the luciferase reporter assay and is expressed as the fold of the relative luciferase activity relative to that in the control tumor cells. (n = 4, data are mean± SD. * P < 0.05, ** P < 0.01).

Techniques Used: Expressing, Activity Assay, Transfection, Luciferase, Reporter Assay

EPA-induced Cx43 expression in combination with 5-FU exerted cytotoxic effects on B16F10 cells. (A) EPA-treated or control cells were transfected with Cx43 shRNA or control plasmids. The expression of Cx43 was determined by Western blotting in B16F10 cells. (B) Down-regulation-Cx43 expression eliminated the cytotoxic effect of 5-FU. EPA-treated or control cells were transfected with Cx43 shRNA or control plasmids. The cells were exposed to 5-FU (0-40 μM) for 24 h followed by determination of their viability by the WST-8 assay. (n = 6, data are mean± SD. * P < 0.05).

Figure Legend Snippet: EPA-induced Cx43 expression in combination with 5-FU exerted cytotoxic effects on B16F10 cells. (A) EPA-treated or control cells were transfected with Cx43 shRNA or control plasmids. The expression of Cx43 was determined by Western blotting in B16F10 cells. (B) Down-regulation-Cx43 expression eliminated the cytotoxic effect of 5-FU. EPA-treated or control cells were transfected with Cx43 shRNA or control plasmids. The cells were exposed to 5-FU (0-40 μM) for 24 h followed by determination of their viability by the WST-8 assay. (n = 6, data are mean± SD. * P < 0.05).

Techniques Used: Expressing, Transfection, shRNA, Western Blot

shRNA:

Article Title: Changes in endothelial connexin 43 expression inversely correlate with microvessel permeability and VE-cadherin expression in endotoxin-challenged lungs
Article Snippet: .. Cx43 shRNA plasmid was purchased from Santa Cruz Biotechnology and packaged into lentiviral vectors by the University of Tennessee Viral Vector Core (Memphis, TN). .. P-lenti-GFP lentiviral vector was from the University of Tennessee Viral Vector Core.

Article Title: Resveratrol Sensitizes Colorectal Cancer Cells to Cetuximab by Connexin 43 Upregulation-Induced Akt Inhibition
Article Snippet: Rabbit IgG, nuclear and cytoplasmic protein extraction kit, and crystal violet were purchased from Beyotime (Shanghai, China). .. Cx43 shRNA plasmid (sc-29276-SH and sc-35091-SH), control shRNA plasmid, Plasmid Transfection Reagent, Plasmid Transfection Medium, mouse anti-Cx43 antibody, and protein A/G PLUS-Agarose were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). pTARGET Mammalian Expression Vector system was purchased from Promega Corporation (Madison, WI, USA). ..

Article Title: Gap junction with MLO-A5 osteoblast-like cell line induces ALP and BSP transcription of 3T3-L1 pre-adipocyte like cell line via Hspb1 while retaining adipogenic differentiation ability.
Article Snippet: In bone tissues, gap junctions form direct links between the cytoplasm of an osteocyte and another adjacent osteocyte or osteoblast, which underlie both bone formation and bone resorption.. We have previously demonstrated that alkaline phosphatase (ALP) and bone sialoprotein (BSP), which are osteoblast markers, were induced in mesenchymal stem cells (MSCs) co-cultured with osteoblast-like cell line.. However, the molecular mechanism of this process has not been fully addressed.

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Cx43, IDO and kynurenine expression varies in different cancer cell lines. (A) The IDO and Cx43 protein level of different cancer cell lines, K1735, B16F10, CT26 and 4T1 were determined by Western blotting. (B) The IDO and Cx43 protein levels in Cx43-knockdown B16F10 cell lines were determined (C) The kynurenine production in Cx43-knockdown B16F10 cell lines were measured by kynurenine assay. (n = 3, data are mean± SD. * P < 0.05, ** P < 0.01, *** P < 0.001).

Journal: International Journal of Medical Sciences

Article Title: The inhibition of indoleamine 2, 3-dioxygenase 1 by connexin 43

doi: 10.7150/ijms.20661

Figure Lengend Snippet: Cx43, IDO and kynurenine expression varies in different cancer cell lines. (A) The IDO and Cx43 protein level of different cancer cell lines, K1735, B16F10, CT26 and 4T1 were determined by Western blotting. (B) The IDO and Cx43 protein levels in Cx43-knockdown B16F10 cell lines were determined (C) The kynurenine production in Cx43-knockdown B16F10 cell lines were measured by kynurenine assay. (n = 3, data are mean± SD. * P < 0.05, ** P < 0.01, *** P < 0.001).

Article Snippet: B16F10 were transfected with Cx43 shRNA plasmids (5 μg) by lipofectamine 2000 (Thermo Scientific, Rockford, IL.

Techniques: Expressing, Western Blot

Resveratrol inhibited IDO expression dose dependently. B16F10 cells (10 6 ) were treated with different concentrations of resveratrol (0-4 μg/ml) for 24 h. (A) The IDO and Cx43 protein levels of resveratrol were determined by Western blotting. (B) B16F10 cells transfected with luciferase gene under the control of Cx43 promoter were treated with resveratrol (0-4 μg/ml) for 24 h. The transcriptional activity of Cx43 was determined by the luciferase reporter assay and is expressed as the fold of the relative luciferase activity relative to that in the control tumor cells. (C) The kynurenine production were measured by kynurenine assay. (D) Supernatants were collected after 24 h resveratrol incubation, and harvested Jurkat cells with equal amount of RPMI. 3 days later, Jurkat cells were stained with trypan blue to measure proliferation rate. (n = 4, data are mean± SD. * P < 0.05, ** P < 0.01).

Journal: International Journal of Medical Sciences

Article Title: The inhibition of indoleamine 2, 3-dioxygenase 1 by connexin 43

doi: 10.7150/ijms.20661

Figure Lengend Snippet: Resveratrol inhibited IDO expression dose dependently. B16F10 cells (10 6 ) were treated with different concentrations of resveratrol (0-4 μg/ml) for 24 h. (A) The IDO and Cx43 protein levels of resveratrol were determined by Western blotting. (B) B16F10 cells transfected with luciferase gene under the control of Cx43 promoter were treated with resveratrol (0-4 μg/ml) for 24 h. The transcriptional activity of Cx43 was determined by the luciferase reporter assay and is expressed as the fold of the relative luciferase activity relative to that in the control tumor cells. (C) The kynurenine production were measured by kynurenine assay. (D) Supernatants were collected after 24 h resveratrol incubation, and harvested Jurkat cells with equal amount of RPMI. 3 days later, Jurkat cells were stained with trypan blue to measure proliferation rate. (n = 4, data are mean± SD. * P < 0.05, ** P < 0.01).

Article Snippet: B16F10 were transfected with Cx43 shRNA plasmids (5 μg) by lipofectamine 2000 (Thermo Scientific, Rockford, IL.

Techniques: Expressing, Western Blot, Transfection, Luciferase, Activity Assay, Reporter Assay, Incubation, Staining

Salmonella (S.C.) inhibited IDO expression. B16F10 cells were treated with various MOI of S.C. (0-100) for 1.5 h and replaced medium with gentimicin-containing medium to kill bacteria. (A) The protein levels of IDO and Cx43 after resveratrol treatment were determined by Western blotting. (B) B16F10 cells transfected with luciferase gene under the control of Cx43 promoter were treated with S.C. (MOI = 0-100) for 24 h. The transcriptional activity of Cx43 was determined by the luciferase reporter assay and is expressed as the fold of the relative luciferase activity relative to that in the control tumor cells. (C) The kynurenine production were measured by kynurenine assay. (D) Supernatants were collected after 24 h resveratrol incubation, and harvested Jurkat cells with equal amount of RPMI. 3 days later, Jurkat cells were stained with trypan blue to measure proliferation rate. (n = 4, data are mean± SD. * P < 0.05, ** P < 0.01, *** P < 0.001).

Journal: International Journal of Medical Sciences

Article Title: The inhibition of indoleamine 2, 3-dioxygenase 1 by connexin 43

doi: 10.7150/ijms.20661

Figure Lengend Snippet: Salmonella (S.C.) inhibited IDO expression. B16F10 cells were treated with various MOI of S.C. (0-100) for 1.5 h and replaced medium with gentimicin-containing medium to kill bacteria. (A) The protein levels of IDO and Cx43 after resveratrol treatment were determined by Western blotting. (B) B16F10 cells transfected with luciferase gene under the control of Cx43 promoter were treated with S.C. (MOI = 0-100) for 24 h. The transcriptional activity of Cx43 was determined by the luciferase reporter assay and is expressed as the fold of the relative luciferase activity relative to that in the control tumor cells. (C) The kynurenine production were measured by kynurenine assay. (D) Supernatants were collected after 24 h resveratrol incubation, and harvested Jurkat cells with equal amount of RPMI. 3 days later, Jurkat cells were stained with trypan blue to measure proliferation rate. (n = 4, data are mean± SD. * P < 0.05, ** P < 0.01, *** P < 0.001).

Article Snippet: B16F10 were transfected with Cx43 shRNA plasmids (5 μg) by lipofectamine 2000 (Thermo Scientific, Rockford, IL.

Techniques: Expressing, Western Blot, Transfection, Luciferase, Activity Assay, Reporter Assay, Incubation, Staining

Resveratrol inhibited IDO expression through upregulating Cx43. B16F10 cells were transfected with Cx43 RNAi. Medium were replaced with 10% FBS contained DMEM. Transfected cells were treated with 4μg/ml resveratrol for 24 h. (A) The IDO and Cx43 protein level of resveratrol were determined by Western blotting. (B) B16F10 or Cx43 knockdown cells transfected with luciferase gene under the control of Cx43 promoter were treated with resveratrol (4 μg/ml) for 24 h. The transcriptional activity of Cx43 was determined by the luciferase reporter assay and is expressed as the fold of the relative luciferase activity relative to that in the control tumor cells. (D) The kynurenine production were measured by kynurenine assay. (C) Supernatants were collected after 24 h resveratrol incubation, and harvested Jurkat cells with equal amount of RPMI. 3 days later, Jurkat cells were stained with trypan blue to measure proliferation rate. (n = 6, data are mean± SD. * P < 0.05).

Journal: International Journal of Medical Sciences

Article Title: The inhibition of indoleamine 2, 3-dioxygenase 1 by connexin 43

doi: 10.7150/ijms.20661

Figure Lengend Snippet: Resveratrol inhibited IDO expression through upregulating Cx43. B16F10 cells were transfected with Cx43 RNAi. Medium were replaced with 10% FBS contained DMEM. Transfected cells were treated with 4μg/ml resveratrol for 24 h. (A) The IDO and Cx43 protein level of resveratrol were determined by Western blotting. (B) B16F10 or Cx43 knockdown cells transfected with luciferase gene under the control of Cx43 promoter were treated with resveratrol (4 μg/ml) for 24 h. The transcriptional activity of Cx43 was determined by the luciferase reporter assay and is expressed as the fold of the relative luciferase activity relative to that in the control tumor cells. (D) The kynurenine production were measured by kynurenine assay. (C) Supernatants were collected after 24 h resveratrol incubation, and harvested Jurkat cells with equal amount of RPMI. 3 days later, Jurkat cells were stained with trypan blue to measure proliferation rate. (n = 6, data are mean± SD. * P < 0.05).

Article Snippet: B16F10 were transfected with Cx43 shRNA plasmids (5 μg) by lipofectamine 2000 (Thermo Scientific, Rockford, IL.

Techniques: Expressing, Transfection, Western Blot, Luciferase, Activity Assay, Reporter Assay, Incubation, Staining

Salmonella (S.C.) inhibited IDO expression through upregulating Cx43. B16F10 cells were transfected with Cx43 RNAi. Medium were replaced with 10% FBS contained DMEM. Transfected cells were treated with Salmonella (MOI=100). (a) The IDO and Cx43 protein level were determined by Western blotting after Salmonell a treatment. (B) The kynurenine production were measured by kynurenine assay. (C) Supernatants were collected after 24 h resveratrol incubation, and harvested Jurkat cells with equal amount of RPMI. 3 days later, Jurkat cells were stained with trypan blue to measure proliferation rate. (n = 6, data are mean± SD. * P < 0.05).

Journal: International Journal of Medical Sciences

Article Title: The inhibition of indoleamine 2, 3-dioxygenase 1 by connexin 43

doi: 10.7150/ijms.20661

Figure Lengend Snippet: Salmonella (S.C.) inhibited IDO expression through upregulating Cx43. B16F10 cells were transfected with Cx43 RNAi. Medium were replaced with 10% FBS contained DMEM. Transfected cells were treated with Salmonella (MOI=100). (a) The IDO and Cx43 protein level were determined by Western blotting after Salmonell a treatment. (B) The kynurenine production were measured by kynurenine assay. (C) Supernatants were collected after 24 h resveratrol incubation, and harvested Jurkat cells with equal amount of RPMI. 3 days later, Jurkat cells were stained with trypan blue to measure proliferation rate. (n = 6, data are mean± SD. * P < 0.05).

Article Snippet: B16F10 were transfected with Cx43 shRNA plasmids (5 μg) by lipofectamine 2000 (Thermo Scientific, Rockford, IL.

Techniques: Expressing, Transfection, Western Blot, Incubation, Staining

Journal: International Journal of Medical Sciences

Article Title: Eicosapentaenoic acids enhance chemosensitivity through connexin 43 upregulation in murine melanoma models

doi: 10.7150/ijms.30889

Figure Lengend Snippet: Effects of EPA on the expression of Cx43 in tumor cells. (A) B16F10 cells were treated with EPA (0-100 μM) for 24 h. The number of cell was measured by the WST-8 assay. (B) The B16F10 cells were treated with of EPA for 24 h. The B16F10 cells were collected and measured for Cx43 by Western blotting. The Immunoblotting assay was repeated three times with similar results.

Article Snippet: The specific shRNA of Cx43 plasmids (sc-35091-SH, Santa Cruz Biotechnology, Inc.) were purchased from Santa Cruz Biotechnology, Inc. B16F10 cells were used lipofectamine 2000 (Invitrogene) to transfected with Cx43 shRNA or control shRNA plasmids .

Techniques: Expressing, Western Blot

Journal: International Journal of Medical Sciences

Article Title: Eicosapentaenoic acids enhance chemosensitivity through connexin 43 upregulation in murine melanoma models

doi: 10.7150/ijms.30889

Figure Lengend Snippet: MAPK inhibitors reduced EPA-induced Cx43 expression. (A) The B16F10 cells were treated with EPA (0-100 μM) for 24h. The cells were lysed and protein expression of ERK, P38, JNK, P-ERK, P-P38, and P-JNK was examined. (B) After treatment of cells with inhibitor for p38 (SB203580) and JNK (SP600125) for 1h, The B16F10 cells were treated with EPA (100 μM) for 24h. The cells were lysed and protein expression of Cx43, P-P38, P-P38, JNK and p-JNK was examined. (C) EPA induced Cx43 transcriptional activity in B16F10 cells. The B16F10 cells transfected with luciferase gene under the control of Cx43 promoter were treated with EPA (0-100 μM) for 24 h. The transcriptional activity of Cx43 was determined by the luciferase reporter assay and is expressed as the fold of the relative luciferase activity relative to that in the control tumor cells. (n = 4, data are mean± SD. * P < 0.05, ** P < 0.01).

Techniques: Expressing, Activity Assay, Transfection, Luciferase, Reporter Assay

Journal: International Journal of Medical Sciences

Article Title: Eicosapentaenoic acids enhance chemosensitivity through connexin 43 upregulation in murine melanoma models

doi: 10.7150/ijms.30889

Figure Lengend Snippet: EPA-induced Cx43 expression in combination with 5-FU exerted cytotoxic effects on B16F10 cells. (A) EPA-treated or control cells were transfected with Cx43 shRNA or control plasmids. The expression of Cx43 was determined by Western blotting in B16F10 cells. (B) Down-regulation-Cx43 expression eliminated the cytotoxic effect of 5-FU. EPA-treated or control cells were transfected with Cx43 shRNA or control plasmids. The cells were exposed to 5-FU (0-40 μM) for 24 h followed by determination of their viability by the WST-8 assay. (n = 6, data are mean± SD. * P < 0.05).

Techniques: Expressing, Transfection, shRNA, Western Blot

Journal: International Journal of Medical Sciences

Article Title: Eicosapentaenoic acids enhance chemosensitivity through connexin 43 upregulation in murine melanoma models

doi: 10.7150/ijms.30889

Techniques: Expressing, Western Blot

Journal: International Journal of Medical Sciences

Article Title: Eicosapentaenoic acids enhance chemosensitivity through connexin 43 upregulation in murine melanoma models

doi: 10.7150/ijms.30889

Techniques: Expressing, Activity Assay, Transfection, Luciferase, Control, Reporter Assay

Journal: International Journal of Medical Sciences

Article Title: Eicosapentaenoic acids enhance chemosensitivity through connexin 43 upregulation in murine melanoma models

doi: 10.7150/ijms.30889

Techniques: Expressing, Control, Transfection, shRNA, Western Blot

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cx43 shrna (Santa Cruz Biotechnology)

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